Monday, 8 August 2016

Doubly Labelled Water - Gold Standard ?

Doubly Labelled Water (DLW) is said to be the "gold standard" of energy measurements but in the world of nutritional research that probably means it's just the least worst option. Most of the materials I've read propose it as a good measure of energy expenditure and it is then combined with body weight and composition changes to determine what the energy intake "must have been" using the calorie hypothesis.

DLW is in the news today as the UK Government's "Nudge Unit" - now operating independently as Behavioural Insights - has been looking at alleged under-reporting of calorie intake in official statistics.

One aspect of accuracy is how repeatable a measure is - if you take the same person and repeat a DLW test in the same circumstances do you get the same answer ? This has been done :-

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3985832/
This apparently is viewed as a good result for reproducibility over a couple of years, but there are many subjects with a difference of about 100 kcal/day between the two measurments are evident. In the sphere of public obesity policy we're often arguing about differences smaller than that.

My biggest query about DLW is whether it has been validated as a way to determine energy intake in a controlled situation where food eaten was independently verified. Most of the published work uses DLW to determine "calories out" and therefore points the finger of under-reporting at anyone reporting a smaller value for "calories in".

The US CALERIE project rides to my rescue with a 7 day calorimeter study snappily titled "Validation study of energy expenditure and intake during calorie restriction using doubly labeled water and changes in body composition". The methodology was to measure energy expenditure by DLW while subjects were contained in a metabolic chamber to provide another measure of energy expenditure. This came out fairly well :
"We found close agreement (R = 0.88) between EE measured in the metabolic chamber and EEDLW during CR. Using the measured respiratory quotient, we found that the mean (±SD) EEDLW was 1934 ± 377 kcal/d and EE measured in the metabolic chamber was 1906 ± 327 kcal/d, ie, a 1.3 ± 8.9% overestimation."
  So DLW reported slightly higher values than the chamber gas analysis but the difference was less than 30 kcal/day.

The next step was to combine the DLW data with changes in body composition and weight, this didn't turn out so well :
"EI calculated from EEDLW and from changes in ES was 8.7 ± 36.7% higher than the actual EI provided during the chamber stay (1596 ± 656 kcal/d)."
 Oops. DLW based calculations estimate a food intake 8.7% higher than that delivered to subjects living in a metabolic chamber. Providing 1430 kcal of food on average led to calculated energy intakes of ~1600 kcal on average using DLW for energy expenditure and weight / DEXA composition to determine the change of energy stores in the body.

So here we see a difference of 170 kcal/day under tightly controlled conditions. Did the subjects sneak in a candy bar each day ? Is the DEXA analysis suspect - it reported a fat-free mass loss of 0.7 kg from the 0.9 kg weight loss. Had all of the 0.9 kg been fat loss the change in energy stores would have risen from a loss of 337 kcal/day to over 1000 kcal/day so this is obviously a sensitive part of the analysis.

 So far I am seeing DLW as a method that might be 100 kcal/day off when estimating energy expenditure, and when combined with body weight and composition changes the error may increase when trying to use this as a measure of energy intake.

A couple of other references for measured intake vs DLW - in female athletes the DLW + bodyweight change estimate was found to be "221 +/- 550 kcal/day in excess of those calculated from food records (2,193 +/- 466 kcal/day)". Note that 221 +/- 550 means a range from +770 to -330 which is a considerable range of uncertainty.

In another study Underweight adults also failed to perform to expectation, overfeeding by 720 kcal/day for 8 weeks failed to increase measured energy expenditure by DLW and 1/3 of the extra energy went unaccounted for during the ~2 kg weight gain.

So where does this leave us ? I don't believe doubly labelled water is sufficiently robust and proven by validation to allow it to be used to determine whether reported energy intake by other means is flawed.

There seems to be too much blind faith in the calorie hypothesis at work - the population got fatter therefore their calorie intake must have gone up even though careful measuring efforts don't show this. Does the science of DLW and assumptions about calories and weight gain really trump detailed reporting of food intake ?

Sunday, 7 August 2016

UK Retailer files low carb health claim

UK retailer Marks & Spencer has filed a claim with the European regulator EFSA for their "Balanced for You" range of ready meals with a limited ratio of carbohydrate to protein.

Wednesday, 16 March 2016

UK to tax production of sugary soft drinks

In the 2016 Budget statement the UK Chancellor (Finance Minister) announced that a levy will be charged on manufacture of sugar sweetened soft drinks. Fruit juice and milk products will be excluded. It is expected to raise £520m (US$730m, €660m) in the first year 2018-19 (UK tax years start on 6th April) declining annually thereafter (£500m, £445m....)

Budget 2016 announces a new soft drinks industry levy targeted at producers and importers of soft drinks that contain added sugar. The levy will be designed to encourage companies to reformulate by reducing the amount of added sugar in the drinks they sell, moving consumers towards lower sugar alternatives, and reducing portion sizes.
Under this levy, if producers change their behaviour, they will pay less tax. The levy is expected to raise £520 million in the first year. The OBR expect that this number will fall over time as the total consumption of soft drinks in scope of the levy drops, in part as a result of producers changing their behaviour and helping consumers to make healthier choices.
According to the British Soft Drinks Association some 45% of carbonated drinks are "regular" rather than "mid" or "no/low" calorie at 6 and 49% respectively.   With a 2014 volume of 6380 million litres of total carbonates that makes 2870 m litres of regular sugar sweetened beverage so the proposed levy looks to cost 18 p/litre (25 US cents).

With the detail to be worked out and various exemptions for small producers etc the exact cost implication to the consumer remains to be seen. The sugar in a litre of 10% SSB probably costs ~4p so this levy is substantial compared to the manufacturer's costs.

There are two bands of levy proposed :-


Soft drinks industry levy
Soft drinks industry levy – The government will introduce a new soft drinks industry levy to be paid by producers and importers of soft drinks that contain added sugar. The levy will be charged on volumes according to total sugar content, with a main rate charge for drink above 5 grams of sugar per 100 millilitres and a higher rate for drinks with more than 8 grams of sugar per 100 millilitres. There will be an exclusion for small operators, and we will consult on the details over the summer, for legislation in Finance Bill 2017 and implementation from April 2018 onwards. (Finance Bill 2017)

Friday, 19 February 2016

Sugar Tax & UK Obesity

This morning the media are awash with PR from a UK Cancer Charity launching a study prepared by a coalition of charities that claims obesity can be reduced by 5% of the UK population by 2025.

I delved into the technical summary as I have an interest in these extrapolations. The claim is that a 20% excise tax levied on sweetened soft drinks could avoid 3.7 million people (about 5%) becoming obese (>=30 BMI) by 2025 and that without the tax obesity will rise from 29% in 2015 to 34% in 2025.

Saturday, 30 January 2016

Everyone's a fruit and nut case

Earlier in the week I awoke to the radio pronouncing that eating fruit was better than eating nuts, or some such. Google turned up a Swedish study "A Randomized Study of the Effects of Additional Fruit and Nuts Consumption on Hepatic Fat Content, Cardiovascular Risk Factors and Basal Metabolic Rate" by Christian Agebratt et al.

This study set out to compare the effects of adding about 500 calories a day of either fruit or nut intake to the existing diet of some young lean active healthy Swedish students. The primary objective was to see if the sugar in the fruits had any adverse effect.

Tuesday, 29 December 2015

Chef succeeds on Atkins Diet

Another personal testimony from the Atkins UK forum :-

Hi Folks,

A have been following some of the threads on this forum for a couple of months now but have not posted until now, so this is my first post here. 

Saturday, 10 October 2015

Atkins Diet success story

Shirley, age 63 and with hypothyroidism, reports a weight loss of 6 stone / 84 lbs / 38 kg in 17 months through following the Atkins diet without exercise and with support from the Atkins UK community forum. That's an average loss of over 1.2 lbs per week.

Here's her story in her own words......